Fig. 3

a One of the rat kidneys in PN group showing focal infarct (depicted by *) at the resection bed (H-E x 15). b Zone of severe ischemia (depicted by **) between regions of infarct (depicted by *) and unaffected renal parenchyma (depicted by ***) (Left: PAS x 100; Right: PAS x 200). c and d Calculations done by the image analysis program. Panel C shows the encircled and measured area of necrosis, while panel D demonstrates the thickness readings of ischemic zone through the perpendicular lines drawn 1 mm apart throughout the entire lesion (A: H-E x 30; B: PAS x 90). e Area of necrosis in PN and RIPC + PN groups (each blue bar indicating a rat belonging to the groups being compared, p= 0.14). f The thickness of ischemic zone in PN and RIPC + PN groups (each red bar indicating a rat belonging to the groups being compared, p= 0.24)